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PTBP1 | Polypyrimidine Tract Binding Protein | 聚嘧啶区结合蛋白 | 关键调控因子

作者:互联网

类似之前的EZH2和HDAC1

 

目的:找PTBP1的target exon(gene)

这个没有生信的办法了,只能根据现有的数据库来找。

 

Google PTBP1 direct target exon

De Novo Prediction of PTBP1 Binding and Splicing Targets Reveals Unexpected Features of Its RNA Recognition and Function

 

待续~

 


 

基本知识回顾:

 

PTBP

 

skipping of polypyrimidine tract–binding protein 1 (PTBP1) exon 9 alters the splicing regulatory activities of PTBP1 and affects the inclusion levels of numerous exons.

During neurogenesis, skipping of exon 9 reduces PTBP1 repressive activity so as to facilitate activation of a brain-specific AS program.

a single exon-skipping event in an RNA binding regulator directs numerous AS changes between species

 

PTBP1 exon 9 in humans, exon 8 in mice

 

逻辑:

有没有exon9,PTBP1的活性有显著差别。有exon9时,PTBP1活性【抑制性】增强,从而导致很多其他基因的exon被抑制,最终抑制了神经系统的发育。【有点绕】

理一下:正常情况下,exon9是skipping的,从而导致PTBP1没有抑制性,所以neuronal exon得以激活。

skipping of PTBP1 exon 9 reduces its repressive activity

 

PTBP1 target exons

 

待续~

 

 

参考文献:

An alternative splicing event amplifies evolutionary differences between vertebrates - Science - 2015

The splicing regulator PTBP1 controls the activity of the transcription factor Pbx1 during neuronal differentiation - eLife - 2015 

A post-transcriptional regulatory switch in polypyrimidine tract-binding proteins reprograms alternative splicing in developing neurons - Genes Dev - 2007

 


读文献,为什么这项研究能发表在Science上,做了些什么工作,有什么核心发现。

 

An alternative splicing event

evolutionary differences

 

问题:

 

 

做了什么:

 

 

 

 

 

标签:splicing,exons,Binding,skipping,exon,Polypyrimidine,Tract,PTBP1,Ptbp1
来源: https://www.cnblogs.com/leezx/p/14476939.html