单细胞数据整合方法 | Comprehensive Integration of Single-Cell Data
作者:互联网
Comprehensive Integration of Single-Cell Data
实在是没想到,这篇seurat的V3里面的整合方法居然发在了Cell主刊。
果然:大佬+前沿领域=无限可能
可以看到bioRxiv上是November 02, 2018发布的,然后Cell主刊June 06, 2019正式发表。
方法的创意应该在2017年底就有了,那时候我才刚来做single cell。
Single-cell transcriptomics has transformed our ability to characterize cell states, but deep biological understanding requires more than a taxonomic listing of clusters.
As new methods arise to measure distinct cellular modalities, a key analytical challenge is to integrate these datasets to better understand cellular identity and function.
Here, we develop a strategy to “anchor” diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities.
After demonstrating improvement over existing methods for integrating scRNA-seq data, we anchor scRNA-seq experiments with scATAC-seq to explore chromatin differences in closely related interneuron subsets and project protein expression measurements onto a bone marrow atlas to characterize lymphocyte populations.
Lastly, we harmonize in situ gene expression and scRNA-seq datasets, allowing transcriptome-wide imputation of spatial gene expression patterns.
Our work presents a strategy for the assembly of harmonized references and transfer of information across datasets.
what's my problem?
标签:cell,datasets,seq,scRNA,Integration,Cell,Data,across 来源: https://www.cnblogs.com/leezx/p/11244731.html